Peripheral nervous system lymphatic vessels: A simple delivery route to promote nerve regeneration

Exp Neurol. 2024 Apr 28:114783. doi: 10.1016/j.expneurol.2024.114783. Online ahead of print.ABSTRACTThe structural and functional features of lymphatic vessels in the peripheral nervous system (pLVs) is still unclear. Here, we clarify the existence of pLVs in rats, PROX1-EGFP transgenic mice and human, and exhibit a clear three-dimensional structure for helping understand its structural features. Moreover, two specific phenotypes of lymphatics endothelial cells (Rnd1Hi LECs and Ccl21Hi LECs) in peripheral nerves are well characterized by single-cell sequencing. Subsequently, the ability of trans-lymphatic delivery to peripheral nerves via pLVs has been dynamically demonstrated. After peripheral nerve injury (PNI), extensive lymphangiogenesis occurs in the lesion area and further enhances the efficiency of retrograde lymphatic-nerve transport. In PNI animal models, subcutaneously footpad-injected exosomes are efficiently delivered to sciatic nerve via pLVs which can promote nerve regeneration. The trans-lymphatic delivery to peripheral nerves via pLVs can subtly bypass BNB which provides an easy and alternative delivery route for PNI treatment.PMID:38688418 | DOI:10.1016/j.expneurol.2024.114783

Source: Experimental Neurology - April 30, 2024 Category: Neurology Authors: Senrui Li Jiangnan Li Xiaoqi Yang Jinsheng Huang Shuai Feng Zhenjun Xie Ningning Yang Yuanyi Wang Nan Zhou Source Type: research