Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from < em > Melaleuca quinquenervia < /em > Fruits

ACS Omega. 2024 Apr 11;9(16):18516-18525. doi: 10.1021/acsomega.4c00769. eCollection 2024 Apr 23.ABSTRACTThe fruit extract of Melaleuca quinquenervia yielded a total of 19 compounds, including two novel spiro-biflavonoid enantiomers (1a and 1b) and a chalcone derivative (3). Their structures were determined through spectroscopic analysis. The enantiomers of the racemic mixture of compound 1 were successfully resolved into (+)-1 and (-)-1 using chiral-phase HPLC. Single-crystal X-ray diffraction analysis was also used to confirm the structure of 1. The enantiomeric configurations of 1 and 2 were determined through a comparison of the calculated and experimental electronic circular dichroism spectra. Compounds 2 (melanervin), 14 (methyl betulinate), 15 (3-O-acetylbetulinic acid), and 16 (pyracrenic acid) were found to be highly cytotoxic, with compound 16 showing superior growth inhibition of nonsmall cell lung cancer cells (A549 cells) (IC50 2.8 ± 0.1 μM) compared to cisplatin (IC50 3.3 ± 0.0 μM), a positive control chemotherapeutic drug. Both compound 16 and cisplatin were significantly more cytotoxic toward A549 lung cancer cells compared to nontumorigenic Vero E6 cells.PMID:38680310 | PMC:PMC11044245 | DOI:10.1021/acsomega.4c00769
Source: Cancer Control - Category: Cancer & Oncology Authors: Source Type: research