Elucidation of inhibitory effects of bioactive anthraquinones towards formation of DNA advanced glycation end products (DNA-AGEs)

Int J Biol Macromol. 2024 Apr 25:131810. doi: 10.1016/j.ijbiomac.2024.131810. Online ahead of print.ABSTRACTDNA is essential in biological processes as it directs transcription and translation assisting in RNA and protein synthesis. Extended periods of elevated blood glucose levels cause non-enzymatic DNA glycation, which results in the formation of DNA-AGEs and the production of free radicals, causing structural perturbation of DNA. In this work, we have investigated the glycation of calf thymus DNA and examined its inhibition by two anthraquinone derivatives, purpurin, and aloin. Ribose sugar served as the glycating agent inducing non-enzymatic glycation of DNA and subsequent DNA-AGEs formation. We use UV-vis and fluorescence spectroscopy to characterize DNA-AGE formation in vitro. Circular dichroism (CD) spectroscopy was used to observe the structural disruption of DNA caused by glycation. The changes in AGEs fluorescence intensity and melting temperature (Tm) were measured to assess the inhibition of glycation process by aloin and purpurin. These derivatives demonstrated inhibitory effects through the binding to glycating sites or by scavenging free radicals generated during glycation. The current study elucidates the inhibitory actions of aloin and purpurin on DNA glycation, suggesting their possible applications in mitigating the adverse consequences linked to increased ribose concentrations.PMID:38677669 | DOI:10.1016/j.ijbiomac.2024.131810

Source: International Journal of Biological Macromolecules - April 27, 2024 Category: Biochemistry Authors: Sana Quraishi Sadia Nudrat Kalpana Kumari Erica W M Marboh Kripamoy Aguan Atanu Singha Roy Source Type: research