Impact of fed-batch process intensification on the productivity and product quality of two CHO cell lines expressing unique novel molecular format proteins

In this study, the impact of a high inoculation density (HID) fed-batch process on the productivity and product quality attributes of two CHO cell lines expressing unique NMFs, a monospecific antibody with an Fc-fusion protein and a bispecific antibody, compared to low inoculation density (LID) platform fed-batch processes was evaluated. It was observed that an intensified platform fed-batch process increased product concentrations by 33 and 109% for the two uniquely structured complex proteins in a shorter culture duration while maintaining similar product quality attributes to traditional fed-batch processes.PMID:38653840 | DOI:10.1007/s00449-024-02997-3

Source: Bioprocess and Biosystems Engineering - April 23, 2024 Category: Biomedical Engineering Authors: Nicolas Q Wolnick Marissa R Dickson Thaddaeus A Webster Roger P Connolly Nancy Fernandes Vesela Encheva Hunter Crittenden Jessica Hodgkins Brian C Hadley Gabriella Palermo Shannon J Hendrick Roy A Newell Genevieve Gray Christian Siltanen Julia Armstrong B Source Type: research