Genes, Vol. 15, Pages 549: A Bidirectional Non-Coding RNA Promoter Mediates Long-Range Gene Expression Regulation

Genes, Vol. 15, Pages 549: A Bidirectional Non-Coding RNA Promoter Mediates Long-Range Gene Expression Regulation Genes doi: 10.3390/genes15050549 Authors: Carlos Alberto Peralta-Alvarez Hober Nelson Núñez-Martínez Ángel Josué Cerecedo-Castillo Augusto César Poot-Hernández Gustavo Tapia-Urzúa Sylvia Garza-Manero Georgina Guerrero Félix Recillas-Targa Recent evidence suggests that human gene promoters display gene expression regulatory mechanisms beyond the typical single gene local transcription modulation. In mammalian genomes, genes with an associated bidirectional promoter are abundant; bidirectional promoter architecture serves as a regulatory hub for a gene pair expression. However, it has been suggested that its contribution to transcriptional regulation might exceed local transcription initiation modulation. Despite their abundance, the functional consequences of bidirectional promoter architecture remain largely unexplored. This work studies the long-range gene expression regulatory role of a long non-coding RNA gene promoter using chromosome conformation capture methods. We found that this particular bidirectional promoter contributes to distal gene expression regulation in a target-specific manner by establishing promoter–promoter interactions. In particular, we validated that the promoter–promoter interactions of this regulatory element with the promoter of distal gene BBX contribute to modulating the trans...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research
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