Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes. The clonal variability in gene copy numbers, integration loci a...

Source: Microbial Cell Factories - April 20, 2024 Category: Microbiology Authors: Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder and Xavier Garcia-Ortega Tags: Research Source Type: research