Probing Protein Complexes Composition, Stoichiometry, and Interactions by Peptide-Based Mass Spectrometry

Adv Exp Med Biol. 2024;3234:41-57. doi: 10.1007/978-3-031-52193-5_4.ABSTRACTThe characterization of a protein complex by mass spectrometry can be conducted at different levels. Initial steps regard the qualitative composition of the complex and subunit identification. After that, quantitative information such as stoichiometric ratios and copy numbers for each subunit in a complex or super-complex is acquired. Peptide-based LC-MS/MS offers a wide number of methods and protocols for the characterization of protein complexes. This chapter concentrates on the applications of peptide-based LC-MS/MS for the qualitative, quantitative, and structural characterization of protein complexes focusing on subunit identification, determination of stoichiometric ratio and number of subunits per complex as well as on cross-linking mass spectrometry and hydrogen/deuterium exchange as methods for the structural investigation of the biological assemblies.PMID:38507199 | DOI:10.1007/978-3-031-52193-5_4

Source: Advances in Experimental Medicine and Biology - March 20, 2024 Category: Research Authors: Gianluca Degliesposti Source Type: research