Chemical constituents of Rubia tibetica Hook. f. from Tibetan medicine and cytotoxic activity evaluation

Fitoterapia. 2024 Apr 14:105961. doi: 10.1016/j.fitote.2024.105961. Online ahead of print.ABSTRACTTwo unprecedented quinone compounds Rubiaxylm A (1) and Rubiaxylm B (2), along with fifteen known anthraquinones (3-17) were isolated and characterized from the roots of Rubia tibetica in Tibetan medicine. Their structures were identified through comprehensive analyses of 1D/2D NMR as well as HR-ESIMS data. Furthermore, all separated compounds were evaluated for their cytotoxic activity on A549, Caco-2, MDA-MB-231 and Skov-3 cell lines. In particular, compound 2 effectively inhibited MDA-MB-231 cells with an IC50 value of 8.15 ± 0.20 μM. Subsequently, the anti-tumor mechanism of 2 was investigated by flow cytometry, JC-1 staining, cell scratching and cell colony. These results indicated that compound 2 could inhibit the proliferation of MDA-MB-231 cells by arresting cells in the G1 phase.PMID:38626855 | DOI:10.1016/j.fitote.2024.105961

Source: Fitoterapia - April 16, 2024 Category: Biochemistry Authors: Min-Min Gu Qing Li Yu Zhang Hong-Wei Wu Yuan-Ling Shao Hong-Ping Han Zhi-Xin Liao Source Type: research