ROLE OF NITRIC OXIDE AND SIGNALING PATHWAYS MODULATING THE STIMULATORY EFFECT OF SNAKE VENOM SECRETORY PLA < sub > 2 < /sub > S ON NON-OPSONIZED ZYMOSAN PHAGOCYTOSIS BY MACROPHAGES

Toxicon. 2024 Apr 11:107716. doi: 10.1016/j.toxicon.2024.107716. Online ahead of print.ABSTRACTThe phagocytic activity of macrophages activated with MT-II, a Lys-49 PLA2 homolog, and MT-III, an Asp-49 PLA2, from Bothrops asper snake venom, was investigated in this study using a pharmacological approach. Stimulating thioglycollate-elicited macrophages with both venom components enhanced their ability to phagocytose non-opsonized zymosan particles. MT-II and MT-III-induced phagocytosis was drastically inhibited by pretreating cells with L-NAME, aminoguanidine or L-NIL, cNOS or iNOS inhibitors, or with ODQ (sGC inhibitor) or Rp-cGMPS (PKG inhibitor). These results indicate that the NO/sGC/GMP/PKG pathway plays an essential role in the β-glucan-mediated phagocytosis induced in macrophages by these venom-secretory PLA2s.PMID:38614247 | DOI:10.1016/j.toxicon.2024.107716

Source: Toxicon - April 13, 2024 Category: Toxicology Authors: Juliana Pavan Zuliani Jos é María Gutiérrez Catarina Teixeira Source Type: research