Genes, Vol. 15, Pages 502: The Impact of Blood Sample Processing on Ribonucleic Acid (RNA) Sequencing

This study aims to investigate the extent and mechanism of the impact of sample selection and processing on ribonucleic acid (RNA) sequencing. RNA from PBMCs and blood samples was investigated in this study. The integrity of this RNA was measured under different storage times. All the samples underwent high-throughput sequencing for comprehensive evaluation. The differentially expressed genes and their potential functions were analyzed after the samples were placed at room temperature for 0h, 4h and 8h, and different feature changes in these samples were also revealed. The sequencing results showed that the differences in gene expression were higher with an increased storage time, while the total number of genes detected did not change significantly. There were five genes showing gradient patterns over different storage times, all of which were protein-coding genes that had not been mentioned in previous studies. The effect of different storage times on seemingly the same samples was analyzed in this present study. This research, therefore, provides a theoretical basis for the long-term consideration of whether sample processing should be adequately addressed.

Source: Genes - April 17, 2024 Category: Genetics & Stem Cells Authors: Zhiyu Liu Tinglan Ouyang Yuwei Yang Yuqi Sheng Huajuan Shi Quanjun Liu Yunfei Bai Qinyu Ge Tags: Article Source Type: research