Three new constituents from the stems of < em > Knema globularia < /em > and their < em > α < /em > -glucosidase inhibitory activity

Nat Prod Res. 2024 Apr 13:1-9. doi: 10.1080/14786419.2024.2341994. Online ahead of print.ABSTRACTThree new metabolites (1-3) were isolated from the stems of Knema globularia, along with five known compounds, including kaempferol (4), quercetin (5), isovanillic acid (6), protocatechuic acid (7), and gallic acid (8). Their structures were deduced using NMR spectroscopic, mass spectrometric analyses, and literature data. The absolute configurations of 1-3 were established by electronic circular dichroism (ECD) spectroscopy. α-Glucosidase inhibitory activities of those compounds were evaluated using a spectrophotometric method, compounds 1-3 showed very strong effects towards α-glucosidase with IC50 values 1.59, 0.58 and 1.37 µM, respectively (the positive control, acarbose, IC50 93.63 µM). Simultaneously, enzyme kinetics study indicated that 2 was a mix-type inhibitor. 2 interacted well in the active site of α-glucosidase enzyme, primarily through hydrogen bonds and hydrophobic interactions.PMID:38613424 | DOI:10.1080/14786419.2024.2341994

Source: Natural Product Research - April 13, 2024 Category: Biochemistry Authors: Thi-Kim-Dung Le Thuc-Huy Duong Warinthorn Chavasiri Source Type: research