Synergistic effects of graphene microgrooves and electrical stimulation on M2 macrophage polarization

In this study, we prepared a graphene free-standing substrate with 20 μm microgrooves using capillary forces induced by water evaporation. Subsequently, we established an ES cell culture platform for macrophage cultivation by integrating a self-designed multi-well chamber cell culture device. We observed that graphene microgrooves, in combination with ES, significantly reduce cell spreading area and circularity. Results from immunofluorescence, ELISA, and flow cytometry demonstrate that the synergistic effect of graphene microgrooves and ES effectively promotes macrophage M2 phenotypic polarization. Finally, RNA sequencing results reveal that the synergistic effects of ES and graphene microgrooves inhibit the macrophage actin polymerization and the downstream PI3K signaling pathway, thereby influencing the phenotypic transition. Our results demonstrate the potential of graphene-based microgrooves and ES to synergistically modulate macrophage polarization, offering promising applications in regenerative medicine.PMID:38603832 | DOI:10.1016/j.bbrc.2024.149911

Source: Biochemical and Biophysical Research communications - April 11, 2024 Category: Biochemistry Authors: Yan Yan Yilin Zhang Kexin Li Yudong Li Wei Qian Wenbo Zhang Yue Wang Wensheng Ma Linhao Li Source Type: research