Small-Angle X-Ray Scattering for Macromolecular Complexes

Adv Exp Med Biol. 2024;3234:163-172. doi: 10.1007/978-3-031-52193-5_11.ABSTRACTSmall angle X-ray scattering (SAXS) is a versatile technique that can provide unique insights in the solution structure of macromolecules and their complexes, covering the size range from small peptides to complete viral assemblies. Technological and conceptual advances in the last two decades have tremendously improved the accessibility of the technique and transformed it into an indispensable tool for structural biology. In this chapter we introduce and discuss several approaches to collecting SAXS data on macromolecular complexes, including several approaches to online chromatography. We include practical advice on experimental design and point out common pitfalls of the technique.PMID:38507206 | DOI:10.1007/978-3-031-52193-5_11

Source: Advances in Experimental Medicine and Biology - March 20, 2024 Category: Research Authors: Stephanie Hutin Mark D Tully Martha Brennich Source Type: research