Sensors, Vol. 24, Pages 2420: Detection of Biomolecules Using Solid-State Nanopores Fabricated by Controlled Dielectric Breakdown

Sensors, Vol. 24, Pages 2420: Detection of Biomolecules Using Solid-State Nanopores Fabricated by Controlled Dielectric Breakdown Sensors doi: 10.3390/s24082420 Authors: Peng Cheng Candong Zhao Qinjie Pan Zijian Xiong Qi Chen Xiangshui Miao Yuhui He Nanopore sensor technology is widely used in biomolecular detection due to its advantages of low cost and easy operation. In a variety of nanopore manufacturing methods, controlled dielectric breakdown has the advantages of a simple manufacturing process and low cost under the premise of ensuring detection performance. In this paper, we have made enhancements to the applied pulses in controlled dielectric breakdown and utilized the improved dielectric breakdown technique to fabricate silicon nitride nanopores with diameters of 5 to 15 nm. Our improved fabrication method offers the advantage of precise control over the nanopore diameter (±0.4 nm) and enhances the symmetry of the nanopore. After fabrication, we performed electrical characterization on the nanopores, and the IV characteristics exhibited high linearity. Subsequently, we conducted detection experiments for DNA and protein using the prepared nanopores to assess the detection performance of the nanopores fabricated using our method. In addition, we also give a physical model of molecule translocation through the nanopores to give a reasonable explanation of the data processing results.
Source: Sensors - Category: Biotechnology Authors: Tags: Article Source Type: research