One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity

This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation of CRISPR/Cas12a, enabling exceptional signal amplification. This approach maximizes the simplicity of the detection method, offering a promising avenue for molecular disease diagnosis.PMID:38591968 | DOI:10.1039/d4cc00825a

Source: Chemical Communications - April 9, 2024 Category: Chemistry Authors: Jianguo Xu Haidong Yang Zhuqi Sui Xinyue Yuan Lee Jia Longhua Guo Source Type: research