Genes, Vol. 15, Pages 476: Identification and Expression Analysis of the WOX Transcription Factor Family in Foxtail Millet (Setaria italica L.)

Genes, Vol. 15, Pages 476: Identification and Expression Analysis of the WOX Transcription Factor Family in Foxtail Millet (Setaria italica L.)
Genes doi: 10.3390/genes15040476
Authors:
Lizhang Nan
Yajun Li
Cui Ma
Xiaowei Meng
Yuanhuai Han
Hongying Li
Mingjing Huang
Yingying Qin
Xuemei Ren

WUSCHEL-related homeobox (WOX) transcription factors are unique to plants and play pivotal roles in plant development and stress responses. In this investigation, we acquired protein sequences of foxtail millet WOX gene family members through homologous sequence alignment and a hidden Markov model (HMM) search. Utilizing conserved domain prediction, we identified 13 foxtail millet WOX genes, which were classified into ancient, intermediate, and modern clades. Multiple sequence alignment results revealed that all WOX proteins possess a homeodomain (HD). The SiWOX genes, clustered together in the phylogenetic tree, exhibited analogous protein spatial structures, gene structures, and conserved motifs. The foxtail millet WOX genes are distributed across 7 chromosomes, featuring 3 pairs of tandem repeats: SiWOX1 and SiWOX13, SiWOX4 and SiWOX5, and SiWOX11 and SiWOX12. Collinearity analysis demonstrated that WOX genes in foxtail millet exhibit the highest collinearity with green foxtail, followed by maize. The SiWOX genes primarily harbor two categories of cis-acting regulatory elements: Stress response and plant hormone response. Notably, prominent hormones triggering res...

Source: Genes - April 10, 2024 Category: Genetics & Stem Cells Authors: Lizhang Nan Yajun Li Cui Ma Xiaowei Meng Yuanhuai Han Hongying Li Mingjing Huang Yingying Qin Xuemei Ren Tags: Article Source Type: research