Probing Effect of 6  MeV Electron Beam Irradiation on Haemoglobin Protein Using Spectroscopic Techniques

Dose Response. 2024 Apr 1;22(2):15593258241240233. doi: 10.1177/15593258241240233. eCollection 2024 Apr-Jun.ABSTRACTIn this work, we study the effect of 6 MeV electron beam irradiation on the physicochemical properties of lyophilized Human Haemoglobin A (HbA). Electron beams generated from Race Track Microtron accelerator with energy 6 MeV were used to irradiate HbA at fluences of 5 × 1014 e-/cm2 and 10 × 1014 e-/cm2. Pristine and electron beam irradiated HbA were characterized using UV-visible and Fourier transform infrared spectroscopy (FTIR) spectroscopy. The interfacial tension of the aqueous solutions of HbA are also analysed by pendant drop method. Absorbance intensity, % transmittance and interfacial tension decrease with fluence. The peak position of the Soret band (λsoret = 404 nm) remains unaffected by the fluences. FTIR spectroscopy confirms the changes in the secondary structure of the haemoglobin. In the amide band I, the percentage of α-helix reduced from 8% to 1%, and an increase in β-sheet (19% to 29%) and β helix (6.3% to 15%) is observed. Interfacial tension decreases from 46.0 mN/m and 44.0 mN/m with increase in irradiation dose. These finding provides realistic guideline for biological cells exposure to electron beam radiation doses.PMID:38576528 | PMC:PMC10989046 | DOI:10.1177/15593258241240233
Source: Dose Response - Category: Drugs & Pharmacology Authors: Source Type: research