Culturable bacteria in clouds at R éunion, tropical island

AbstractThe viable bacterial assemblages in clouds at R éunion Island (Indian Ocean) were examined through culture-based approach. A total of 176 isolates were recovered from 15 independent cloud events collected during 3 field campaigns, and identified to the species level through full length 16S rRNA gene sequencing. As often in atmospheric samples, A lpha-, Beta- and Gamma-proteobacteria dominated, along with Actinobacteria, Firmicutes, and Bacteroidetes, depicting these as the backbone of the global aeromicrobiome. A comparative analysis with similar work from a cloud sampling site in Central France revealed site-specificities, and numerous com mon species. These latter included members ofPseudomonas,Sphingomonas,Bacillus,Staphylococcus,Rhodococcus and others, whose such widespread presence in clouds supports the existence of a pan-atmospheric microbiome. This also confirms that cultures remain powerful methods in the description of the viable microbial diversity by allowing deep taxonomic affiliation.
Source: Aerobiologia - Category: Environmental Health Source Type: research