Complete genome analysis of a novel narnavirus in sweet viburnum (Viburnum odoratissimum)

In this study, we discovered a new positive-sense RNA virus originating fromViburnum odoratissimum, which we named"Vo narna-like virus". The complete genome of Vo narna-like virus is 3,451 nt in length with an open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRP) protein. Phylogenetic analysis placed this virus within the betanarnavirus clade, sharing 53.63% amino acid sequence identity with its closest relative, Qingdao RNA virus 2. The complete sequence of the virus was confirmed by rapid amplification of cDNA ends (RACE) and Sanger sequencing. Small interfering RNA (siRNA) analysis indicated that this virus interacts with the RNA interference (RNAi) pathway ofV. odoratissimum. This is  the first report of a narnavirus inV. odoratissimum.

Source: Archives of Virology - April 5, 2024 Category: Virology Source Type: research