High-Throughput Cell-Based Screening of Small Molecule KRAS Signaling Inhibitors Using a Homogeneous Time-Resolved Fluorescence (HTRF) Assay

Methods Mol Biol. 2024;2797:271-285. doi: 10.1007/978-1-0716-3822-4_20.ABSTRACTWith recent advances proving that effective inhibition of KRAS is possible, there have been significant efforts made to develop inhibitors of specific mutant alleles. Here we describe a detailed protocol that employs homogeneous time-resolved fluorescence (HTRF) to identify compounds acting on KRAS signaling in malignant cell lines. This method allows for high-throughput, cell-based screens of large compound libraries for the development of RAS-targeted therapeutics.PMID:38570467 | DOI:10.1007/978-1-0716-3822-4_20
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research