Quality Control of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel

Methods Mol Biol. 2024;2797:337-350. doi: 10.1007/978-1-0716-3822-4_24.ABSTRACTIsogenic H/N/KRAS-less mouse embryonic fibroblast (MEF) cell lines have been developed to assist in cell-based assays of RAS inhibitors. The quality control assessment of a panel of these isogenic MEFs is described here, with a focus on ensuring the proper insertion of the desired mutant RAS transgene, a determination of gene copy number, and an investigation of potential off-target mutations which could lead to phenotypes which are undesired in downstream experiments. Using this suite of quality control tools, a MEF cell line can be readily validated, and researchers can be assured of the rationale for an observed phenotype.PMID:38570471 | DOI:10.1007/978-1-0716-3822-4_24
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research