Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene

In this study, we present a new approach employing a multi-intron containing Cas9 gene plus multiple gRNAs separated with tRNA sequences to target the phytoene desaturase gene in both 'Carrizo' citrange and 'Duncan' grapefruit. Notably, using this unified vector significantly boosted editing efficiency in both citrus varieties, showcasing mutations in all three designated targets. The implementation of this multiplex gene editing system with a multi-intron-containing Cas9 plus a gRNA-tRNA array demonstrates a promising avenue for efficient citrus genome editing, equipping us with potent tools in the ongoing battle against several diseases such as canker and huanglongbing.PMID:38564120 | DOI:10.1007/s11248-024-00380-2

Source: Transgenic Research - April 2, 2024 Category: Genetics & Stem Cells Authors: Poulami Sarkar Jorge Santiago Vazquez Mingxi Zhou Amit Levy Zhonglin Mou Vladimir Orbovi ć Source Type: research