Diagnosis of thrombotic thrombocytopenic purpura: Easy-to-use fiber-optic surface plasmon resonance immunoassays for automated ADAMTS13 antigen and conformation evaluation

CONCLUSIONS: Both ADAMTS13 antigen and conformation assays were transferred onto automated, easy-to-use FO-SPR technology displaying potent analytical sensitivity and reproducibility. ADAMTS13 antigen and conformation were determined for healthy donors and acute iTTP patients showing strong correlation with ELISA reference. Introducing FO-SPR technology in clinical context could support routine diagnosis of acute iTTP patients, notably when ADAMTS13 activity fluctuates between 10-20%.PMID:38554935 | DOI:10.1016/j.jtha.2024.03.012

Source: Thrombosis and Haemostasis - March 30, 2024 Category: Hematology Authors: Quintijn Bonnez Charlotte Dekimpe Tim Bekaert Edwige Tellier Gilles Kaplanski B érangère S Joly Agn ès Veyradier Paul Coppo Jeroen Lammertyn Claudia Tersteeg Simon F De Meyer Karen Vanhoorelbeke Source Type: research