Agro-ecosystem of honeybees as source for native probiotic yeasts

AbstractProbiotic microorganisms are used to improve the health and wellness of people and the research on this topic is of current relevance and interest. Fifty-five yeasts, coming from honeybee ’s ecosystem and belonging toCandida,Debaryomyces,Hanseniaspora,Lachancea,Metschnikowia,Meyerozyma,Starmerella andZygosacchromyces genera and related different species, were evaluated for the probiotic traits. The resistance to gastrointestinal conditions, auto-aggregation, cell surface hydrophobicity or biofilm formation abilities as well as antimicrobial activity against common human pathogenic bacteria were evaluated. The safety analysis of strains was also carried out to exclude any possible negative effect on the consumer ’s health. The influence of proteinase treatment of living yeasts and their adhesion to Caco-2 cells were also evaluated. The greatest selection occurred in the first step of survival at the acidic pH and in the presence of bile salts, where more than 50% of the strains were unable to survive. Equ ally discriminating was the protease test which allowed the survival of only 27 strains belonging to the speciesHanseniaspora guilliermondii,Hanseniaspora uvarum,Metschnikowia pulcherrima,Metschnikowia ziziphicola,Meyerozyma caribbica,Meyerozyma guilliermondii,Pichia kluyveri,Pichia kudriavzevii andPichia terricola. An integrated analysis of the results obtained allowed the detection of seven yeast strains with probiotic aptitudes, all belonging to theMeyerozyma g...
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research