Standardized production of hPSC-derived cardiomyocyte aggregates in stirred spinner flasks

We present a protocol for achieving efficient generation of hPSC-CM aggregates in suspension culture, emphasizing process simplicity, robustness and GMP compliance. The strategy promotes clinical translation and other applications that require large numbers of CMs.

Source: Nature Protocols - March 28, 2024 Category: Biomedical Science Authors: Nils Kriedemann Wiebke Triebert Jana Teske Mira Mertens Annika Franke Kevin Ullmann Felix Manstein Lika Drakhlis Alexandra Haase Caroline Halloin Ulrich Martin Robert Zweigerdt Source Type: research