Human seminal extracellular vesicles enhance endometrial receptivity through leukemia inhibitory factor

Endocrinology. 2024 Mar 22:bqae035. doi: 10.1210/endocr/bqae035. Online ahead of print.ABSTRACTSeminal extracellular vesicles (EVs) contain different subgroups which have diverse effects on sperm function. However, the effect of seminal EVs-especially its subgroups, on the endometrial receptivity was largely unknown. Here, we found that the seminal EVs could be divided into high-density EV (EV-H)、medium density EV (EV-M) and low-density EV (EV-L), after purification using iodixanol. Then we demonstrated that EV-H could promote the expression and secretion of leukemia inhibitor factor (LIF) in human endometrial cells. In EV-H-treated endometrial cells, we identified 1274 differentially expressed genes (DEGs). DEGs were enriched in cell adhesion and AKT, STAT3 pathways. Therefore, we illustrated that EV-H enhanced the adhesion of human choriocarcinoma JAr cell spheroids to endometrial cells through the LIF-STAT3 pathway. Collectively, our findings indicated that seminal EV-H could regulate endometrial receptivity through LIF pathway, which would provide novel insights into male fertility.PMID:38518755 | DOI:10.1210/endocr/bqae035
Source: Endocrinology - Category: Endocrinology Authors: Source Type: research