Establishment of a simple, sensitive, and specific ASFV detection method based on Pyrococcus furiosus argonaute

This study reports on a new nucleic acid assay method, termed REPD, which uses recombinase-aided amplification and restriction endonuclease-assisted Pyrococcus furiosus argonaute (PfAgo) detection. One-pot REPD was developed for the detection of ASFV. The one-pot REPD could detect a single copy of ASFV nucleic acid and showed no cross-reactivity with other pathogens. Detection in clinical samples was 100% consistent with the results of real-time PCR analysis. The results showed that the one-pot REPD assay is convenient, sensitive, specific, and potentially adaptable to the detection of ASFV. In summary, this study highlights a novel method that can be employed for the detection of pathogens.PMID:38520983 | DOI:10.1016/j.bios.2024.116230
Source: Biosensors and Bioelectronics - Category: Biotechnology Authors: Source Type: research