Rapid and sensitive detection of SARS-CoV-2 IgM through luciferase luminescence on an automatic platform

Int J Biol Macromol. 2024 Mar 16:130964. doi: 10.1016/j.ijbiomac.2024.130964. Online ahead of print.ABSTRACTSARS-CoV-2 has brought a global health crisis worldwide. IgM is an early marker in sera after the infections, and the detection of IgM is crucial to assist diagnosis and evaluate the vaccination clinically. Herein, we developed an automated platform to identify IgM against SARS-CoV-2 in sera. Streptavidin-magnetic beads were utilized to bind to a biotinylated anti-IgM antibody, which was employed to capture IgM in sera. RBD fused luciferase hGluc was employed to label the trapped IgM against RBD and the signal of luminescence of hGluc with the substrate of coelenterazine corresponded to the amount of SARS-CoV-2 IgM conjugated to the magnetic beads. An appropriate cut-off value of the designed method was defined by a set of negative samples and positive samples with 100 % sensitivity and 100 % specificity. Through serial dilution of a positive sample, it was found that the method has a better sensitivity than ELISA. The application to determine IgM against SARS-CoV-2 demonstrated a good performance of the method. The developed system can complete the analysis of SARS-CoV-2 IgM within 25 min. Through the substitution of RBD antigen with antigens of other pathogens in this platform, the automated detection of IgM against the corresponding pathogens can be realized.PMID:38499123 | DOI:10.1016/j.ijbiomac.2024.130964

Source: International Journal of Biological Macromolecules - March 18, 2024 Category: Biochemistry Authors: Yibing Zhang Yun Zhang Wenhao Zhou Ping He Xueni Sun Junhua Li Hongping Wei Junping Yu Source Type: research