Golgi apparatus-targeting fluorescent probe for the imaging of superoxide anion (O < sub > 2 < /sub > < sup > •- < /sup > ) in living cells during ferroptosis

Anal Chim Acta. 2024 Apr 15;1298:342410. doi: 10.1016/j.aca.2024.342410. Epub 2024 Feb 26.ABSTRACTFerroptosis is an emerging iron-dependent oxidative cell death type, and recently has been demonstrated to show close relation with Golgi apparatus (GA). Exploring the fluctuation of superoxide anion (O2•-) level in GA during ferroptosis is of great significance to profoundly study the biological functions of GA in ferroptosis. Here, we present a GA-targeting probe (N-GA) to monitor cellular O2•- during ferroptosis. N-GA employed a triflate group and a tetradecanoic amide unit as the recognition site for O2•- and GA-targeting unit, respectively. After the response of N-GA to O2•-, the triflate unit of N-GA converted into hydroxyl group with strong electron-donating ability, generating bright green fluorescence under UV light. N-GA exhibited excellent sensitivity and selectivity towards O2•-. Fluorescence imaging results showed that N-GA could be applied as a GA-targeting probe to monitor cellular O2•-. The stimulation of cells with PMA and rotenone could result in the massive generation of endogenous O2•- in GA. Erastin-induced ferroptosis can markedly induce the increase of O2•- level in GA. Similar to Fer-1 and DFO, dihydrolipoic acid (DHLA) and rutin were demonstrated to inhibit the enormous production of O2•- in GA of the living cells during ferroptosis.PMID:38462334 | DOI:10.1016/j.aca.2024.342410

Source: Analytica Chimica Acta - March 10, 2024 Category: Chemistry Authors: Jia Chang Yan Wang Xiuqi Kong Baoli Dong Tao Yue Source Type: research