Quantitative phosphoproteomics explain cryopreservation-induced reductions in ram sperm motility

This study used phosphorylated TMT quantitative proteomics to explore regulation of epigenetic modifications in frozen-thawed ram sperm. This experiment demonstrated that ram sperm freezing affects phosphorylation site modifications of proteins, especially those related to functions such as sperm motility and energy production. Furthermore, it is important to link functions of phosphorylated proteins with changes in sperm quality after freezing and thawing, and to clarify intrinsic reasons for sperm quality changes, which is of great importance for elucidating mechanisms of sperm freezing damage. Based on these protein markers and combined with cryoprotectant design theory, it provides a theoretical basis and data reference to study sperm cryoprotectants.PMID:38438079 | DOI:10.1016/j.jprot.2024.105153

Source: Journal of Proteomics - March 4, 2024 Category: Biochemistry Authors: Shengqin Zang Xiaorui Yang Jiangfeng Ye Xianhong Mo Guangbin Zhou Yi Fang Source Type: research