REG γ mitigates radiation-induced enteritis by preserving mucin secretion and sustaining microbiome homeostasis

Am J Pathol. 2024 Feb 27:S0002-9440(24)00077-4. doi: 10.1016/j.ajpath.2024.02.008. Online ahead of print.ABSTRACTRadiation-induced enteritis, a significant concern in abdominal radiation therapy, is closely associated with gut microbiota dysbiosis. The critical mucus layer plays a pivotal role in preventing the translocation of commensal and pathogenic microbes. While the significant expression of REGγ in intestinal epithelial cells is well established, its role in modulating mucus layer and gut microbiota remains enigmatic. Current study revealed notable changes in gut microorganisms and metabolites in irradiated mice lacking REGγ, as opposed to wild-type mice. Concomitant with gut microbiota dysbiosis, REGγ deficiency facilitated the infiltration of neutrophils and macrophages, thereby exacerbating intestinal inflammation following irradiation. Furthermore, fluorescence in situ hybridization assays unveiled an augmented proximity of bacteria to intestinal epithelial cells in REGγ knockout mice post-irradiation. Mechanistically, deficiency of REGγ led to diminished goblet cell populations and reduced expression of key goblet cell markers, Muc2 and Tff3, observed in both murine models, mini-gut organoid system and human intestinal goblet cells, indicating the intrinsic role of REGγ within goblet cells. Interestingly, while administration of broad-spectrum antibiotics didn't impact the alteration of goblet cell numbers and MUC2 secretion, it did effectively attenuate inf...
Source: The American Journal of Pathology - Category: Pathology Authors: Source Type: research