RTCB deficiency triggers colitis in mice by influencing the NF- κB and Wnt/β-catenin signaling pathways

In this study, we employ a conditional knockout mouse model to investigate the effects of RTCB depletion on the colon and the potential molecular mechanisms. We assess the efficiency and phenotype of Rtcb knockout using PCR, western blot analysis, histological staining, and immunohistochemistry. Compared with the control mice, the Rtcb-knockout mice exhibit compromised colonic barrier integrity and prominent inflammatory cell infiltration. In the colonic tissues of Rtcb-knockout mice, the protein levels of TNF-α, IL-8, and p-p65 are increased, whereas the levels of IKKβ and IκBα are decreased. Moreover, the level of GSK3β is increased, whereas the levels of Wnt3a, β-catenin, and LGR5 are decreased. Collectively, our findings unveil a close association between RTCB and colonic tissue homeostasis and demonstrate that RTCB deficiency can lead to dysregulation of both the NF-κB and Wnt/β-catenin signaling pathways in colonic cells.PMID:38425245 | DOI:10.3724/abbs.2023279

Source: Acta Biochimica et Biophysica Sinica - March 1, 2024 Category: Biochemistry Authors: Peiyan Liu Ruitao Zhang Xiaotong Song Xiaohua Tian Yichao Guan Licheng Li Mei He Chengqiang He Naizheng Ding Source Type: research