Viruses, Vol. 16, Pages 395: Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
Viruses, Vol. 16, Pages 395: Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
Viruses doi: 10.3390/v16030395
Authors:
Tempone
Zezza-Ramalho
Borely
Pitaluga
Brazil
Brandão-Filho
Pessoa
Bruno
Carvalho-Costa
Salomón
Volf
Burleigh
Aguiar
Traub-Cseko
Sandflies are known vectors of leishmaniasis. In the Old World, sandflies are also vectors of viruses while little is known about the capacity of New World insects to transmit viruses to humans. Here, we relate the identification of RNA sequences with homology to rhabdovirus nucleocapsids (NcPs) genes, initially in the Lutzomyia longipalpis LL5 cell lineage, named NcP1.1 and NcP2. The Rhabdoviridae family never retrotranscribes its RNA genome to DNA. The sequences here described were identified in cDNA and DNA from LL-5 cells and in adult insects indicating that they are transcribed endogenous viral elements (EVEs). The presence of NcP1.1 and NcP2 in the L. longipalpis genome was confirmed in silico. In addition to showing the genomic location of NcP1.1 and NcP2, we identified another rhabdoviral insertion named NcP1.2. Analysis of small RNA molecules derived from these sequences showed that NcP1.1 and NcP1.2 present a profile consistent with elements targeted by primary piRNAs, while NcP2 was restricted to the degradation profile. The presence of NcP1.1 and NcP2 was investigated in sandfly popu...
Source: Viruses - Category: Virology Authors: Tempone Zezza-Ramalho Borely Pitaluga Brazil Brand ão-Filho Pessoa Bruno Carvalho-Costa Salom ón Volf Burleigh Aguiar Traub-Cseko Tags: Article Source Type: research