GSE197769 High coverage single cell RNA-Seq of pooled MCF7 and HEK293-ZRT cells to discover expression patterns of the LINE1 retrotransposon

Contributor : Victoria BelancioSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe performed high coverage scRNA-Seq on a pool of MCF7 and HEK293-ZRT cells in a 9:1 ratio, respectively, using a total of 853 cells in this experiment. Detection of mobile element expression at the locus-specific level requires high sequencing depth. Here, MCF7 and HEK293-ZRT single cells have been sequenced to a sufficient level to quanitfy LINE1 expression in single cells and compare expression differences between individual cells.Single-cell RNA sequencing was carried out on individual cells using the 10X Genomics Chromium ™ Single Cell 3’ Library& Gel Bead Kit v3 and 150 cycle kit. 750 cells were targeted for sequencing.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research