Random and site-specific radiolabeling of [89Zr]Zr-DFO-anti-PD-L1-mAb iPET tracer

In this study, random and site-specific radiolabeling of89Zr-iPET tracers were investigated. Traditional lysine-based conjugation and enzyme-based GlyCLICK® kit method were used to prepare DFO-anti-PD-L1-mAb conjugates with a chelator-to-antibody ratio (CAR) of 0 –7 and 2.0, respectively. Then, conjugates with different CAR levels were radiolabeled with89Zr to obtain randomly or site-specifically radiolabeled89Zr-iPET tracer.
Source: Journal of Radioanalytical and Nuclear Chemistry - Category: Nuclear Medicine Source Type: research