Lower basal and postprandial muscle protein synthesis after 2  weeks single‐leg immobilization in older men: No protective effect of anti‐inflammatory medication

AbstractMuscle inactivity may reduce basal and postprandial muscle protein synthesis (MPS) rates in humans. Anti-inflammatory treatment alleviates the MPS impairments in younger individuals. The present study explored the influence of nonsteroidal anti-inflammatory drugs (NSAIDs) upon MPS during a period of inactivity in older humans. Eighteen men (age 60 –80 years) were allocated to ibuprofen (1200 mg/day, Ibu) or control (Plc) groups. One lower limb was cast immobilized for 2 weeks. Postabsorptive and postprandial MPS was measured before and after the immobilization by L-[ring-13C6]-phenylalanine infusion. The protein expression of select anabolic signaling molecules was investigated by western blot. Basal (0.038  ± 0.002%/h and 0.039 ± 0.005%/h, Plc and Ibu, respectively) and postprandial (0.064 ± 0.004%/h and 0.067 ± 0.010%/h, Plc and Ibu, respectively) MPS rate were higher pre-immobilization compared to basal (0.019 ± 0.005%/h and 0.020 ± 0.010%/h, Plc and Ibu, respectively) and postprandial (0.033 ± 0.005%/h and 0.037 ± 0.006%/h, Plc and Ibu, respectively) MPS rate post-immobilization (p <  0.001). NSAID treatment did not affect the suppression of MPS (p >  0.05). The anabolic signaling were in general reduced after immobilization (p <  0.05). These changes were unaffected by NSAID treatment (p >  0.05). Basal and postprandial MPS dropped markedly after 2 weeks of lower limb immobilization. NS...

Source: Physiological Reports - February 26, 2024 Category: Physiology Authors: K. Dideriksen, S. Reitelseder, A. P. Boesen, M. Zillmer, J. Agergaard, M. Kjaer, L. Holm Tags: ORIGINAL ARTICLE Source Type: research