The use of single-molecule FRET for the characterization of Holliday junctions containing human telomeric DNA: a methodological approach for nanoscale distance, mobility, and stability measurements

We describe in detail the setup and optimization of the single-molecule fluorescence microscope, along with data acquisition and analysis procedures. Furthermore, we address potential challenges and provide troubleshooting strategies to ensure successful d ata collection and interpretation. The presented methodology serves as a valuable resource for researchers aiming to employ smFRET to study Holliday junctions and other biomolecular systems at the nanoscale, paving the way for further advancements in the field of single-molecule biophysics.
Source: Medicinal Chemistry Research - Category: Chemistry Source Type: research