Single-cell RNA sequencing of cells from fresh or frozen tissue reveals a signature of freezing marked by heightened stress and activation

Eur J Immunol. 2024 Feb 2:e2350660. doi: 10.1002/eji.202350660. Online ahead of print.ABSTRACTThawing of viably frozen human tissue T cells, ILCs, and NK cells and subsequent single-cell RNA sequencing reveals that recovery of cellular subclusters is variably impacted. While freeze-thawing does not alter the transcriptional profiles of cells, it upregulates genes and gene pathways associated with stress and activation.PMID:38304946 | DOI:10.1002/eji.202350660

Source: European Journal of Immunology - February 2, 2024 Category: Allergy & Immunology Authors: Christopher T Stamper Anne Marchalot Christopher A Tibbitt Whitney Weigel Mattias Jangard Jakob Theorell Jenny Mj ösberg Source Type: research