Increased expression of complement C3c, iC3b and cells containing CD11b or CD14 in experimentally induced psoriatic lesion

In this study, the tape-stripping technique was used to induce the development of Köbner reaction in 18 subjects with psoriasis. Eight subjects developed the positive reaction. To study the early cellular changes, skin biopsies were taken at the baseline and subsequent time points of 2 hours, 1 day, 3 days and 7 days for the immunostaining of complement C3c, iC3b, and cells expressing complement receptor 3 (CD11b/CD18; a receptor of iC3b) or CD14. The results show that the positive Köbner reaction associated with rapid (2h-1d) and sustained (3-7d) increase in the expression of epidermal C3c and iC3b and dermal C3c. In addition, there was a positive correlation between CD11b+ and CD14+ cells in baseline and 2h-1d biopsies with a subsequent increase in CD11b+ and CD14+ cells in 3-7d biopsies in the Köbner-positive group. In the Köbner-negative group, only a transient increase in epidermal iC3b at 2h-1d, as well as rapid (2h-1d) and sustained increase (3-7d) in dermal iC3b and CD14+ cells, was observed. In experiments with cultured monolayer keratinocytes, a slight cell damage already at 30 mJ/cm2 ultraviolet B irradiation led to increased expression of C3c, but not iC3b. Therefore, there are marked differences between Köbner groups in respect to the expression of C3c, iC3b and cells expressing CD11b or CD14. Of note is the rapid and sustained increase in epidermal C3c and iC3b in the positive Köbner reaction.PMID:38310540 | DOI:10.1093/cei/uxae009
Source: Clinical and Developmental Immunology - Category: Allergy & Immunology Authors: Source Type: research