Characterizing the detection of inactivated Mycoplasma hyopneumoniae DNA in the respiratory tract of pigs

AbstractA positiveMycoplasma hyopneumoniae PCR result in a clinical specimen may eventually represent the mere detection of non-viable bacteria, complicating the diagnostic interpretation. Thus, the objective of this study was to evaluate the PCR detection of non-viableM. hyopneumoniae and its residual cell-free DNA in live pigs. Pigs were inoculated with either active or inactivatedM. hyopneumoniae and were sampled for up to 14  days.Mycoplasma hyopneumoniae was not detected by PCR at any timepoint in pigs inoculated with the inactivated bacterium, suggesting that in healthy pigs, the non-viableM. hyopneumoniae DNA was rapidly sensed and cleared.
Source: Veterinary Research - Category: Veterinary Research Source Type: research