DGK5 β-derived phosphatidic acid regulates ROS production in plant immunity by stabilizing NADPH oxidase

Cell Host Microbe. 2024 Feb 2:S1931-3128(24)00015-5. doi: 10.1016/j.chom.2024.01.011. Online ahead of print.ABSTRACTIn plant immunity, phosphatidic acid (PA) regulates reactive oxygen species (ROS) by binding to respiratory burst oxidase homolog D (RBOHD), an NADPH oxidase responsible for ROS production. Here, we analyze the influence of PA binding on RBOHD activity and the mechanism of RBOHD-bound PA generation. PA binding enhances RBOHD protein stability by inhibiting vacuolar degradation, thereby increasing chitin-induced ROS production. Mutations in diacylglycerol kinase 5 (DGK5), which phosphorylates diacylglycerol to produce PA, impair chitin-induced PA and ROS production. The DGK5 transcript DGK5β (but not DGK5α) complements reduced PA and ROS production in dgk5-1 mutants, as well as resistance to Botrytis cinerea. Phosphorylation of S506 residue in the C-terminal calmodulin-binding domain of DGK5β contributes to the activation of DGK5β to produce PA. These findings suggest that DGK5β-derived PA regulates ROS production by inhibiting RBOHD protein degradation, elucidating the role of PA-ROS interplay in immune response regulation.PMID:38309260 | DOI:10.1016/j.chom.2024.01.011
Source: Cell Host and Microbe - Category: Microbiology Authors: Source Type: research