Identification, characterization and application of M16AT, a novel organic solvent-tolerant (R)-enantio-selective types IV amine transaminase from Mycobacterium sp. ACS1612 †

Chembiochem. 2024 Feb 13:e202300812. doi: 10.1002/cbic.202300812. Online ahead of print.ABSTRACTChiral amines widely exist in a variety of natural products and bioactive molecules. The catalytic asymmetric synthesis of chiral amines by R-selective amine transaminases (R-ATAs) has been realized. Thus, identification and characterization of novel R-ATAs will make great potential contributions to the industrial production of enantiopure chiral amines and amino acids. Here, new R-selective amine transaminases (R-ATAs) from Mycobacterium sp. ACS1612 (M16AT) using in-silico prediction combined with genome and protein database has been obtained. A two-step simple purification process could yield high concentration of purity enzyme, suggesting a low costs for industrial application. Additionally, the new identified and characterized R-ATAs displayed a broad substrate spectrum and strong organic solvent-tolerance. Moreover, the synthetic applicability of M16AT has been demonstrated by the asymmetric synthesis of (R)-Fendiline of 1-phenylethan-1-amine.PMID:38351400 | DOI:10.1002/cbic.202300812
Source: Chembiochem - Category: Biochemistry Authors: Source Type: research