Cancers, Vol. 16, Pages 785: Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA) Samples from Advanced Non-Small Cell Lung Cancer for Whole Genome, Whole Exome and Comprehensive Panel Sequencing
Cancers, Vol. 16, Pages 785: Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA) Samples from Advanced Non-Small Cell Lung Cancer for Whole Genome, Whole Exome and Comprehensive Panel Sequencing
Cancers doi: 10.3390/cancers16040785
Authors:
David Fielding
Vanessa Lakis
Andrew J. Dalley
Haarika Chittoory
Felicity Newell
Lambros T. Koufariotis
Ann-Marie Patch
Stephen Kazakoff
Farzad Bashirzadeh
Jung Hwa Son
Kimberley Ryan
Daniel Steinfort
Jonathan P. Williamson
Michael Bint
Carl Pahoff
Phan Tien Nguyen
Scott Twaddell
David Arnold
Christopher Grainge
Andrew Pattison
David Fairbairn
Shailendra Gune
Jemma Christie
Oliver Holmes
Conrad Leonard
Scott Wood
John V. Pearson
Sunil R. Lakhani
Nicola Waddell
Peter T. Simpson
Katia Nones
Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is often the only source of tumor tissue from patients with advanced, inoperable lung cancer. EBUS-TBNA aspirates are used for the diagnosis, staging, and genomic testing to inform therapy options. Here we extracted DNA and RNA from 220 EBUS-TBNA aspirates to evaluate their suitability for whole genome (WGS), whole exome (WES), and comprehensive panel sequencing. For a subset of 40 cases, the same nucleic acid extraction was sequenced using WGS, WES, and the TruSight Oncology 500 assay. Genomic features were compared between sequencing platforms and compared with those report...
Source: Cancers - Category: Cancer & Oncology Authors: David Fielding Vanessa Lakis Andrew J. Dalley Haarika Chittoory Felicity Newell Lambros T. Koufariotis Ann-Marie Patch Stephen Kazakoff Farzad Bashirzadeh Jung Hwa Son Kimberley Ryan Daniel Steinfort Jonathan P. Williamson Michael Bint Carl Pahoff Phan Ti Tags: Article Source Type: research
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