Cancers, Vol. 16, Pages 785: Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA) Samples from Advanced Non-Small Cell Lung Cancer for Whole Genome, Whole Exome and Comprehensive Panel Sequencing

Cancers, Vol. 16, Pages 785: Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA) Samples from Advanced Non-Small Cell Lung Cancer for Whole Genome, Whole Exome and Comprehensive Panel Sequencing Cancers doi: 10.3390/cancers16040785 Authors: David Fielding Vanessa Lakis Andrew J. Dalley Haarika Chittoory Felicity Newell Lambros T. Koufariotis Ann-Marie Patch Stephen Kazakoff Farzad Bashirzadeh Jung Hwa Son Kimberley Ryan Daniel Steinfort Jonathan P. Williamson Michael Bint Carl Pahoff Phan Tien Nguyen Scott Twaddell David Arnold Christopher Grainge Andrew Pattison David Fairbairn Shailendra Gune Jemma Christie Oliver Holmes Conrad Leonard Scott Wood John V. Pearson Sunil R. Lakhani Nicola Waddell Peter T. Simpson Katia Nones Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is often the only source of tumor tissue from patients with advanced, inoperable lung cancer. EBUS-TBNA aspirates are used for the diagnosis, staging, and genomic testing to inform therapy options. Here we extracted DNA and RNA from 220 EBUS-TBNA aspirates to evaluate their suitability for whole genome (WGS), whole exome (WES), and comprehensive panel sequencing. For a subset of 40 cases, the same nucleic acid extraction was sequenced using WGS, WES, and the TruSight Oncology 500 assay. Genomic features were compared between sequencing platforms and compared with those report...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research