Evaluation of a single Eporatio ® micro‐dose in urine and dried blood spots

A micro-dose (15  IU/kg) of the recombinant human erythropoietin Eporatio® given to male subjects resulted in the following findings from the urine and dried blood spot (DBS) analyses: Eporatio® was detected in all urine and DBS samples 10–36 h after micro-dose administration; after 72 h, 40% of urine samp les and 60% of DBS samples were suspicious for the presence of Eporatio®; and there was no significant difference between Mitra® VAMS and Capitainer® B50 DBS devices in the EPO signal intensities. AbstractRecombinant human erythropoietin (rhEPO) has been abused as a performance enhancer in sports for several years, but with advancements in detection methods, even micro-doses can be detected in dried blood spot (DBS) samples. Here, we present the results from an Eporatio ® (epoetin theta) micro-dose administration study to detect rhEPO in DBS samples. Five healthy male volunteers received a 15 IU/kg subcutaneous dose of Eporatio®. Urine and DBS samples (Mitra® VAMS and Capitainer® B50) were collected 1, 10, 24, 36, 48 and 72 h after drug administration. Afte r 1 h, all urine samples were negative for rhEPO, whereas 40% of DBS samples were considered suspicious. All samples between 10 and 48 h were suspicious for the presence of Eporatio®, except one urine sample that was negative at 48 h. After 72 h, 40% of urine samples and 60% of DBS samples were suspicious and would have proceeded to a confirmation analysis. DBS is an efficient complementary ...
Source: Drug Testing and Analysis - Category: Drugs & Pharmacology Authors: Tags: SHORT COMMUNICATION Source Type: research