Viruses, Vol. 16, Pages 235: Detection of Chikungunya Virus RNA in Oral Fluid and Urine: An Alternative Approach to Diagnosis?

Viruses, Vol. 16, Pages 235: Detection of Chikungunya Virus RNA in Oral Fluid and Urine: An Alternative Approach to Diagnosis? Viruses doi: 10.3390/v16020235 Authors: Leile Camila Jacob-Nascimento Moyra M. Portilho Rosângela O. Anjos Patrícia S. S. Moreira Christine Stauber Scott C. Weaver Uriel Kitron Mitermayer G. Reis Guilherme S. Ribeiro To evaluate whether oral fluids (OF) and urine can serve as alternative, non-invasive samples to diagnose chikungunya virus (CHIKV) infection via RT-qPCR, we employed the same RNA extraction and RT-qPCR protocols on paired serum, OF and urine samples collected from 51 patients with chikungunya during the acute phase of the illness. Chikungunya patients were confirmed through RT-qPCR in acute-phase sera (N = 19), IgM seroconversion between acute- and convalescent-phase sera (N = 12), or IgM detection in acute-phase sera (N = 20). The controls included paired serum, OF and urine samples from patients with non-arbovirus acute febrile illness (N = 28) and RT-PCR-confirmed dengue (N = 16). Nine (47%) of the patients with positive RT-qPCR for CHIKV in sera and two (17%) of those with CHIKV infection confirmed solely via IgM seroconversion had OF positive for CHIKV in RT-qPCR. One (5%) patient with CHIKV infection confirmed via serum RT-qPCR was positive in the RT-qPCR performed on urine. None of the negative control group samples were positive. Although OF may serve as an alternative sample for diagnosing acute chikung...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research