Bacterial genome engineering using CRISPR-associated transposases

Nature Protocols, Published online: 12 January 2024; doi:10.1038/s41596-023-00927-3This protocol provides extensive guidelines and detailed steps to generate novel bio-engineered bacterial strains using CRISPR-associated transposase (CAST) systems, with available plasmids and standard molecular biology techniques.

Source: Nature Protocols - January 12, 2024 Category: Biomedical Science Authors: Diego Rivera Gelsinger Phuc Leo H. Vo Sanne E. Klompe Carlotta Ronda Harris H. Wang Samuel H. Sternberg Source Type: research