Recent advances in genome engineering by CRISPR technology

BMB Rep. 2023 Dec 6:6038. Online ahead of print.ABSTRACTDue to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the offtarget problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized.PMID:38053294

Source: BMB Reports - December 6, 2023 Category: Biochemistry Authors: Youngsik Lee Yeounsun Oh Seung Hwan Lee Source Type: research