Examining the Capacity of Human U1 snRNA Variants to Facilitate Pre-mRNA Splicing

In this study, we found that placing the variants in the context of the regulatory elements of the RNU1-1 gene improves expression of many variants to levels comparable to the canonical U1 snRNA. Application of a previously established HeLa cell based minigene reporter assay to examine the capacity of the vU1 snRNAs to support pre-mRNA splicing revealed that even though the exogenously expressed variant snRNAs were enriched in the nucleus, only a few had a measurable effect on splicing.PMID:38164604 | DOI:10.1261/rna.079892.123

Source: RNA - January 2, 2024 Category: Genetics & Stem Cells Authors: Jason Wong Ryan Yellamaty Christina Gallante Ethan Lawrence William Martelly Shalini Sharma Source Type: research