Deleterious effect of chronic high-dose ethanol intake on biomechanical bone properties and periodontal status

AbstractTo evaluate the effect of high-graduation chronic ethanol (EtOH) intake on bone and periodontal tissues of rats. Male Wistar rats (250  g) were divided into two groups ofn = 12 each one. EtOH (5 ml of 3 g/kg) was administered to the experimental group by gastric gavage twice a day for 20 days and the control group received water under the same conditions. The rats were euthanized and used to perform biochemical determination in plasma and gingival tissue, and histological and biomechanical studies in the femur and mandibular tissues. Alcohol increased both TNFα (p <  0.01) and PGE2 (p <  0.05) in plasma and gingiva (p <  0.05) as compared to controls. In addition, EtOH increased the alveolar bone loss as evidenced by the increased distance between the cement enamel junction and the alveolar crest (p <  0.01), the lower % of interradicular bone expressed as bone area/total area (B.Ar/T.Ar,p <  0.05) and the larger periodontal space (p <  0.05), as compared to controls. Likewise, the mandibular microtomographic analysis in alcoholized rats revealed a lower % of interradicular bone volume/total volume (BV/TV,p <  0.05), greater trabecular separation (p <  0.05) and greater % trabecular porosity (p <  0.05) than controls. No biomechanical alteration was observed in lower jaws, while the femur of alcoholized rats presented a decrease in the structural bone properties (p <  0.001), as a systemic ...
Source: Odontology - Category: Dentistry Source Type: research