Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions

This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchymal transition (EMT) markers, estrogen-metabolizing proteins, and estrogen/progesterone receptors in ihAMEC was similar to the expression seen in primary cells. In addition, the response of ihAMEC under estrogen treatment and Lipopolysaccharide intervention is similar to primary cells. The clonogenic ability of ihAMEC was lower than tumor cells and did not form tumors in tumorigenicity assays. Thus, ihAMEC can be used as in vitro cellular model for pathogenesis and drug development studies regarding AM.PMID:38134573 | DOI:10.1016/j.tice.2023.102284

Source: Tissue and Cell - December 22, 2023 Category: Cytology Authors: Zilu Wang Fengxin Cui Yinuo Chen Hongyun Liu Yiran Zhang Yaxin Shi Yinuo Zhang Yanfei Wang Na Liang Li Xu Ying Liu Zhiyong Liu Wei Shi Source Type: research